UNIT Nine BIOTECHNOLOGY

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UNIT Nine BIOTECHNOLOGY

Chapter Nine Biotechnology: Principles and Processes

Ever since the days of Rene Descartes, the French philosopher, mathematician and biologist of seventeenth century, all human knowledge especially natural sciences were directed to develop technologies which add to the creature comforts of human lives, as also value to human life. The whole approach to understanding natural phenomena became anthropocentric. Physics and chemistry gave rise to engineering, technologies and industries which all worked for human comfort and welfare. The major utility of the biological world is as a source of food. Biotechnology, the twentieth century off-shoot of modern biology, changed our daily life as its products brought qualitative improvement in health and food production. The basic principles underlying biotechnological processes and some applications are highlighted and discussed in this unit.

Herbert Boyer was born in nineteen thirty-six and brought up in a corner of western Pennsylvania where railroads and mines were the destiny of most young men. He completed graduate work at the University of Pittsburgh, in nineteen sixty-three, followed by three years of post-graduate studies at Yale.

In nineteen sixty-six, Boyer took over assistant professorship at the University of California at San Francisco. By nineteen sixty-nine, he performed studies on a couple of restriction enzymes of the E. coli bacterium with especially useful properties. Boyer observed that these enzymes have the capability of cutting DNA strands in a particular fashion, which left what has became known as `sticky ends' on the strands. These clipped ends made pasting together pieces of DNA a precise exercise.

This discovery, in turn, led to a rich and rewarding conversation in Hawaii with a Stanford scientist named Stanley Cohen. Cohen had been studying small ringlets of DNA called plasmids and which float about freely in the cytoplasm of certain bacterial cells and replicate independently from the coding strand of DNA. Cohen had developed a method of removing these plasmids from the cell and then reinserting them in other cells. Combining this process with that of DNA splicing enabled Boyer and Cohen to recombine segments of DNA in desired configurations and insert the DNA in bacterial cells, which could then act as manufacturing plants for specific proteins. This breakthrough was the basis upon which the discipline of biotechnology was founded.

CHAPTER Nine

BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

Nine point one Principles of Biotechnology

Nine point three Processes of Recombinant DNA Technology

Nine point one PRINCIPLES OF BIOTECHNOLOGY

Nine point two TOOLS OF RECOMBINANT DNA TECHNOLOGY

Nine point two point one Restriction Enzymes

Nine point two point three Competent Host (For Transformation with Recombinant DNA)

Nine point three PROCESSES OF RECOMBINANT DNA TECHNOLOGY

Nine point three point one Isolation of the Genetic Material (DNA)

Nine point three point two Cutting of DNA at Specific Locations

Nine point three point three Amplification of Gene of Interest using PCR

Nine point three point four Insertion of Recombinant DNA into the Host Cell or Organism

Nine point three point five Obtaining the Foreign Gene Product

Nine point three point six Downstream Processing

SUMMARY

EXERCISES

Overview

The chapter outlines the foundational principles of biotechnology, detailing the significance of genetic engineering and its applications in modifying living organisms for human benefit. It explores key developments, including the discovery of restriction enzymes and the creation of recombinant DNA.

Key Points

1Biotechnology utilizes living organisms and enzymes to produce useful products
2Genetic engineering allows for the precise alteration of genetic material
3The discovery of restriction enzymes was crucial for recombinant DNA technology
4Recombinant DNA techniques enable the production of proteins in bacterial cells
5Applications of biotechnology include gene therapy, DNA vaccines, and genetically modified organisms.

Details

Authors: Herbert Boyer
Category: Biology and Natural Sciences

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