C. Factors involved in Fixation
Two. Types of Fixatives According to Composition
C. Histochemical Fixatives
Six. General Precautions in Handling and Fixation of Specimens
Eight. Difficulties Caused by Improper Fixation
Ten. Fixation by Tissue Component
A. Two Classes of Fixatives
TYPES OF FIXATIVES ACCORDING TO COMPOSITION
TYPES OF FIXATIVE ACCORDING TO ACTION
GENERAL PRECAUTIONS IN HANDLING AND FIXATION OF SPECIMENS
CHARACTERISTICS OF GOOD FIXATIVES
DIFFICULTIES CAUSED BY IMPROPER FIXATION
FIXATION BY TISSUE COMPONENT
Fixation for Enzyme Histochemistry
Fixation for Immunofluorescence
Four. Methanol-Acetone Fixation
Six. Methanol-Ethanol Mix Fixation
Eight. Paraformaldehyde-Triton Fixation
HISTOPATHOLOGIC AND CYTOLOGIC TECHNIQUES LEC
B. Ten percent Formol-Salide
Three. Metallic Fixatives
Three. Heidenhain's Susa Solution
C. Brasils's Alcoholic Picroformol Fixative
B. Methyl Alcohol one hundred percent
D. Ethyl Alcohol seventy to one hundred percent
Type of Chemical Fixatives
Two. Precipitating - Alcoholic Fixatives
Four. Glacial Acetic Acid Fixatives
Six. Osmium Tetroxide Fixatives
B. Ten percent Formol-Saline
C. Ten percent Neutral Buffered Formalin or Phosphate Buffered Formalin (pH seven)
D. Formol-Corrosive (Formol Sublimate)
Two. Zenker's Formol (Helly Solution)
Three. Heidenhain's Susa Solution
Two. Regards (Miller's) Fluid
C. Brasil's Alcoholic Picroformol Fixative
GLACIAL ACETIC ACID FIXATIVES
B. Methyl Alcohol one hundred percent
Tissue Preservation: Fixation
C. Isopropyl Alcohol ninety-five percent
D. Ethyl Alcohol (seventy to one hundred percent)
OSMIUM TETROXIDE / OSMIC ACID
HISTOPATHOLOGIC & CYTOLOGIC TECHNIQUES LEC
B. Flemming's Solution without Acetic Acid
TRICHOLOROACETIC ACID (TCA)
Two. Microwave Irradiation
Two Ways in which Microwave Technology is used for Tissue Fixation
Microwave-assisted Fixation